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Kinetic assay for AChE inhibition by 6c. The kinetic assay of AChE inhibition was carried out at 0, 30, 50 and 60 µM of compound 6c. The concentration of ATCI substrate was varied between 10 to 250 µM. The resulting shown Lineweaver-Burk plot generated by the GraphPad Prism 7.03 software proved that compound 6c inhibited the enzyme by mixed-type inhibition mode. [ATCI], substrate concentration (µM); V, reaction velocity (1/V (abs/min)−1).
