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Effects of HM-V on DNCB-induced mast cell infiltration and organ size. (A) Mouse back skin lesions were fixed in 10% formaldehyde, sectioned into 4.5-μm slices, and stained with H&E and Toluidine Blue O. (B) Local lymph node size. (C) Spleen size. (D) Image analysis of lymph node and spleen. Data shown are the average of six or seven samples per group and analyzed using the ImageJ program 1.8.0_172. ## p < 0.05 versus negative control, ** p < 0.05 versus positive control.
