Table 2.

Overview of the NetB and Alpha toxin production of 5 C. perfringens strains with different virulence. All strains were grown in TGY medium for 24 h, after which both the NetB titre (supernatant dilution that induces 50% of haemolysis) and alpha toxin activity (CPA in units/mL) in the supernatants were determined. The virulence of the strains is expressed as % disease induction in an NE in vivo model, relative to the virulence of strain NE18 [23]. Data are expressed as mean ± standard deviation of the mean.

Strain ID	NetB Titre	CPA U/mL	% Disease Induction
JIR12058	588 ± 54	0.048 ± 0.03	154
NE18	466 ± 115	0.102 ± 0.04	100
CP56	337 ± 60	0.119 ± 0.05	80
JIR4866	436 ± 103	0.118 ± 0.03	75
JIR4860	200 ± 57	0.646 ± 0.10	47