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. 2021 Aug 3;10(8):982. doi: 10.3390/pathogens10080982

Figure 1.

Figure 1

Figure 1

Conditioned medium from LMP1-expressing cells enhances the rate of fibroblast motility. (a(i)) The wound healing scratch assay confirms HDFa cell migration is enhanced by overnight culture in conditioned medium taken from LMP1-expressing MDCK cells. Images captured by phase contrast on the EVOS FL digital fluorescence microscope. Results are representative of experiments performed in triplicate. (a(ii)) ImageJ analysis quantified the wound area at set time points. The results depict mean % wound closure over time after scratch (mean ± SD, n = 3). Asterisk indicates result significantly different from the serum-free control * = p < 0.0005. Significant differences were determined using a mixed ANOVA. (b) The Promega CellTiter 96® Aqueous One Solution Cell Proliferation Assay kit confirms the observed effects in (a) arise from enhanced motility and not enhanced proliferation. Results show absorbance after 3 days (mean ± SD, n = 3). Significant differences were determined using a one-way ANOVA. (c) Transwell migration assay further corroborates HDFa recruitment in response to LMP1-conditioned medium. Images were captured using an inverted Leica microscope, with attached Leica MC170 HD camera, and Leica Application Suite (LAS) software). Results are representative of experiments performed in triplicate. (d) ImageJ analysis determined percentage migration. Three representative fields per condition were measured and mean was calculated (mean ± SD, n = 3). Significant differences were determined using the Student t-test.