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. 2021 Aug 3;10(8):982. doi: 10.3390/pathogens10080982

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LMP1 enhances HDFa cellular proliferation over the course of a week. (a) HDFa cells were cultured in conditioned medium from MDCK-pLNSX control cells, LMP1-expressing cells and serum free medium over a 7-day time course prior to cell counting. The results depict cell number (mean ± SD, n = 3). Asterisk indicates significant difference, determined using the Student t-test, from day 1 of the same conditions (p < 0.01). (b) HDFa cells were cultured in conditioned medium from MDCK-pLNSX control cells, LMP1-expressing cells and serum free medium over a 7-day time course prior to trypan blue exclusion assay. The results depict % viable cells (mean ± SD, n = 3). Using the Student t-test no significant differences were found between conditions (p ≥ 0.05).

LMP1 enhances HDFa cellular proliferation over the course of a week. (a) HDFa cells were cultured in conditioned medium from MDCK-pLNSX control cells, LMP1-expressing cells and serum free medium over a 7-day time course prior to cell counting. The results depict cell number (mean ± SD, n = 3). Asterisk indicates significant difference, determined using the Student t-test, from day 1 of the same conditions (p < 0.01). (b) HDFa cells were cultured in conditioned medium from MDCK-pLNSX control cells, LMP1-expressing cells and serum free medium over a 7-day time course prior to trypan blue exclusion assay. The results depict % viable cells (mean ± SD, n = 3). Using the Student t-test no significant differences were found between conditions (p ≥ 0.05).