FIG. 10.
The exonuclease activity of C-terminally truncated p53 is not influenced by okadaic acid treatment. High Five insect cells were infected with recombinant baculovirus coding for the C-terminally truncated p531-360 fragment. The cells were split, and one half was treated with okadaic acid (OA). The p531-360 fragment from both samples was purified by metal affinity chromatography, analyzed by SDS-PAGE (A), and tested for exonuclease activity (B) and sequence-specific DNA binding (C). Numbers on the left in panel A are molecular masses in kilodaltons.