FIG. 2.
wt but not mutant p53 core domain is necessary and sufficient for p53 exonuclease activity. wt (A and C) and MethA (B and D) p53 core fragments (amino acids 80 to 280) were expressed in bacteria and purified by metal affinity chromatography. Column fractions of both proteins were analyzed by SDS-PAGE (A and B) and tested for exonuclease activity by the 3H filter retention assay (C and D). Exonuclease III (lane C) was used as a control. Numbers on the left in panels A and B are molecular masses in kilodaltons.