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. 2021 Aug 27;40:272. doi: 10.1186/s13046-021-02074-7

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Circ-GALNT16 validation and expression in CRC cells and tissues. a Heatmap of top ten upregulated and downregulated expression circRNAs between CRC tissues and adjacent normal tissues according to the circRNA microarray. b The schematic illustration showed the back splicing of circ-GALNT16, and sanger sequence validated the splicing site. c PCR and agarose gel electrophoresis confirmed the circular formation of circ-GALNT16, using divergent and convergent primers in gDNA and cDNA of DLD-1. GAPDH was used as a negative control. d, e. Circ-GALNT16 and linear GALNT16 expression levels were detected after RNase R or actinomycin D treatment in DLD-1. f. Relative circ-GALNT16 expression in 100 CRC tissues and matched adjacent normal tissues. g-h. Relative expression of circ-GALNT16 in different tumor size and tumor stage groups. i. Kaplan-Meier plots of the overall survival of CRC patients with high (n = 50) and low (n = 50) levels of circ-GALNT16. All data are presented as the means ± SD of three independent experiments. ^nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001