Fig. 2.

Circ-GALNT16 suppresses the proliferation of CRC cells in vitro. a, b. CCK8 assays were applied to determine the growth curves of circ-GALNT16 knockdown or overexpression cells. c, d. Colony formation assays were performed to evaluate cell proliferation ability. e, f. EdU assays were performed to assess the cell proliferation ability. g, h. Cell cycle distributions were detected by flow cytometry in circ-GALNT16 knockdown or overexpression cells. i, j. The apoptotic rates were performed and analyzed after cells were treated with 0.5mM H₂0₂ for 4 h. All data are presented as the means ± SD of three independent experiments. **p < 0.01, ***p < 0.001