Figure 1.

24 h-kinetic curves of the HAP dissolution using phytate, alendronate, and etidronate as inhibitors was incubated with nothing (control), phytate, alendronate, or etidronate (1 or 3 µM) to ensure that polyphosphates bound to HAP crystal surfaces. After that, the HAP-adsorbed drug was exposed to acid-driven dissolution. Aliquots were withdrawn at different times during 24 h and analyzed for the dissolved calcium: (A) phytate; (B) alendronate; (C) etidronate. All experiments were performed in triplicate. HAP: hydroxyapatite.