Figure 2.

Effects of chitosan on metabolic signaling protein expression in the liver of diabetic rats with different feeding durations. (A) 6-week feeding duration; (B) 11-week feeding duration. The protein expression for phosphorylated AMPK, AMPK, PPAR-α, PPAR-γ, MTTP, SREBP1c, and β-actin (internal control) was determined by Western blot analysis. The densitometrical quantification is shown. Results are expressed as mean ± S.D. for each group (n = 4–6). The significant difference (p < 0.05) were analyzed by one-way ANOVA and two-tailed Student’s t-test. *: versus C; #: versus DM; @: versus DM + TZD.