Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 21;11(8):856. doi: 10.3390/life11080856

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Western blot analysis of total protein samples for Poly (ADP-ribose) polymerase 1 (PARP) cleavage from monolayer cultured HepG2 and differentiated HepaRG in response to acetaminophen (APAP) treatment after 24 h (top left panel). Total c-Jun protein levels were determined in monolayer cultured differentiated HepaRG after 10 and 15 mM acetaminophen treatment or 15 mM acetaminophen and dabrafenib (10 μM) (top right panel). β-actin and GAPDH were labeled for loading control. Densitometry data represent the intensity of cleaved PARP normalized for β-actin and total c-Jun normalized to GAPDH. For each of the experiments, at least three independent measurements were carried out. * significantly different (p < 0.05) from untreated.