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. 2021 Aug 21;11(8):856. doi: 10.3390/life11080856

Figure 7.

Figure 7

The effect of 3D culture techniques (spheroid and nanofiber) on CYP2E1 mRNA expression and APAP-induced cell death in HepG2 and differentiated HepaRG (a). CYP2E1 mRNA was determined by real-time RT-PCR from both cell lines cultured either in a 2D monolayer or 3D (spheroid or nanofiber). Each CYP2E1 expression is normalized to the expression of the 2D cultured HepG2 line. In the case of HepaRG, CYP2E1 was also monitored throughout the differentiation process (9 and 28 days). Cell death induced by different concentrations of acetaminophen (APAP, 0 mM—untreated, 10 mM, 15 mM, and 20 mM) or 15 mM acetaminophen and inhibitors (zVAD-fmk 40 μM, dabrafenib (Dabr) 10 μM, necrostatin-1 (Nec-1) 50 μM, or liproxstatin-1 (Lipr-1) 1 μM) was measured by the AST assay (b). Data are normalized to untreated (0 mM), and each data point represents the average ± SD of at least three independent experiments. * significantly different (p < 0.05) from untreated (0 mM APAP); # significantly different (p < 0.05) from 15 mM APAP.