In vivo targeting evaluation. In vivo real-time imaging of saline, DiR-HCPT-NS, and DiR-HCPT-NS/CCM in glioma-bearing mice, n = 3 (A). Ex vivo fluorescence images of the brain and other major organs of mice treated intravenously with different DiR-encapsulated nanosuspensions (B). The brain CT tumor localization scan, which indicates that biomimetic nanosuspensions are able to effectively transport drugs to the brain tissue (C). Analysis of the permeability experiment of free DiI and HCPT-NS/CCM on brain tumor site of glioma mice (D). Image J was utilized to quantify the distribution of free DiI and HCPT-NS/CCM in brain of mice that bore intracranial C6 glioma (E). (DiI: red, DAPI: blue, by 40× object lens; The data points represent the mean ± SD. ** indicates p < 0.01).