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HT (Hesperetin) decreased the interaction of ACE2 and the spike protein. FRET assay was performed to determine the interaction between human receptor ACE2 and S protein (A). The in vitro enzymatic activity of TMPRSS2 (B), PLpro (C), and 3CLpro (D) was determined after 1 hr incubation with HT and HD (Hesperidin). Data are shown as mean ± SEM from 3 independent experiments with triplicates. * p < 0.05.
