Figure 2.

Largazole inhibits choroidal vessel outgrowth and has synergic manner with, while it has no impact on existing choroidal vessel. (A) Representative images (upper) and quantitative analysis (lower) of microvessel formation from mouse choroidal explants demonstrating a significant inhibitory effect of Largazole (n = 3 independent experimental groups, n ≥ 6 explants per treatment group). (B) Representative images (upper) and quantitative analysis (lower) of choroidal vessel outgrowth following Largazole treatment as compared to that before the treatment (n = 3 independent experimental groups, n ≥ 6 explants per treatment group). Pre-D2, images were taken after choroid explants have been embedded for 2 days. D4 images were taken after the choroid explants have been embedded for 4 days and treated with vehicle or Largazole for 2 days. Sprouting area increments were calculated used the following equation: (D4 vessel sprouting area / pre-D2 vessel sprouting area) × 100% − 100%. Scale bar: 100 μm. All images shown are representative, and data are presented as means ± s.e.m. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparison test or unpaired, two-tailed Student’s t-test; *** p < 0.001.