Table 1.
Antibodies used in the analysis by flow cytometry.
| No. | Manufacturer | Product | Usage |
|---|---|---|---|
| 1 | BioLegend, CA, USA | PE anti-human CD45 Antibody Clone 2D1 | CD45 |
| 2 | BioLegend | PE Mouse IgG1, κ Isotype Ctrl (FC) Antibody clone MOPC-21 | Isotype |
| 3 | BioLegend | APC anti-human CD44 Antibody Clone BJ18 | CD44 |
| 4 | BioLegend | APC Mouse IgG1, κ Isotype Ctrl Antibody clone MOPC-21 | Isotype |
| 5 | Miltenyi biotec, Bergisch Gladbach, Germany | CD133/1 VioBright 667 mouse IgG1 clone AC133 | CD133 |
| 6 | Miltenyi biotec | VioBright 515 mouse IgG1, Clone IS5-21F5 | Isotype |
| 7 | Stemcell, Canada | ALDEFLUOR kit #01700 | ALDH |
| 8 | Invitrogen, CA, USA | CD326 (EpCAM) Monoclonal Antibody PE-Cyanine7 clone 1B7 | EpCAM |
| 9 | Invitrogen | PE-Cyanine7 mouse/IgG1, kappa clone P3.6.2.8.1 | Isotype |
| 10 | Abcam, Cambridge, United Kingdom | Mouse Anti-Cytokeratin 8 + 18 + 19 antibody clone 2A4 (ab41825) IgG1 | Primary |
| 11 | BioLegend | Brilliant Violet 421™ anti-mouse IgG1 Antibody clone RMG1-1 | Secondary |
| 12 | BioLegend | Alexa Fluor 647 anti-Vimentin Antibody clone 091D3 Mouse IgG2a | Vimentin |
| 13 | BioLegend | Alexa Fluor 647 anti-mouse IgG2a clone RMG2a-62 | Isotype |