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Pylipid-predicted cholesterol binding sites are conserved across membrane conditions and populated with CRAC/CARC motifs. (a) Binding sites are mapped onto the model (left), with cholesterol residency time plotted as a function of membrane cholesterol % (right) for binding sites identified in grooves between TM helices. (b) CRAC/CARC motifs are color-coded by which predicted binding site they are found within, produced using the GPCRdb [74].
