Figure 5.

The melanin synthesis and tyrosinase activity of KCR, KCS, KCL, and KCF extract in α-MSH-stimulated melanocytes. Melanocytes B16F10 were treated with KCR, KCS, KCL, and KCF extract (0.5 mg/mL) for 1 h after being treated to α-MSH (0.5 μM) or non-treatment. These melanocytes were maintained for 48 h. (A) The percentage of viable melanocytes was assessed using the CCK-8 assay. Intracellular melanin content (B) and tyrosinase activity assays (C) were determined. Relative mean values were calculated by normalizing for protein content. * p < 0.05 and ** p < 0.01 (vs. α-MSH-stimulated group). Arbutin (A, 0.5 mg/mL) was used as a positive control. Data are expressed as mean ± SEM (n = 3).