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. 2021 Jul 27;13(8):1143. doi: 10.3390/pharmaceutics13081143

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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UV-Vis spectra acquired during the time-course of the NanoBro synthesis. Green line shows the HEPES-buffered Bro solution. Orange lines show the spectral changes of HAuCl₄ in subsequent times after addition to Bro solution as indicated by the arrow. Gray lines show the increase of absorbance and turbidity resulted from NanoBro formation. The inset shows a snapshot of the HEPES-buffered Bro transparent solution, the yellow HAuCl₄ aqueous solution and the red-wine GNP suspension. The synthesis was carried out at pH 7.0 at the temperature of 40 °C and the spectra were obtained in a quartz cuvette with 1 cm of optical path.