Figure 2.
Characterization of nanoparticles. (a) UV-Vis spectra of NanoBro colloidal suspension produced with HEPES-buffered Bro (line 1), with HEPES and sodium phosphate (line 2), with aqueous solution of Bro (line 3). Green lines 4 and 5 correspond, respectively, to the spectra of HEPES-buffered Bro (0.2 mg/mL) and the supernatant of NanoBro synthesis (line 1) obtained by GNPs precipitation by centrifugation. The inset shows, from left to right snapshots of solutions 1–4. Spectra were obtained 4 days after the synthesis; (b) transmission electron microscopy (TEM) of NanoBro was obtained with magnification of 100,000× and energy of 80 kW; (c) histogram of NanoBro size distribution fitted as lognormal curve by Origin tool calculated a mean diameter of of 5.0 ± 1.3 nm.
