Table 1.
Summary of all animal inoculations and sample collections.
| Animal | n | Sex | Inoculation Route | Animals Per Inoculum Group | Euthanasia Timepoint | Samples Collected from Each Animal | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| ZIKV 41525 | ZIKV PRVABC59 | Sham-Inoculated | Ante-Mortem Samples ** | Non-Reproductive Organs *** | Reproductive Organs | |||||
| Multimammate mouse (Mastomys natalensis) | 6 | F | SC | 2 | 3 | 1 | 5 dpi | Blood, Saliva | Brain, heart, lungs, liver, spleen, kidney, bladder | Ovary |
| 6 | M | 2 | 3 | 1 | Testes, Seminal vesicles | |||||
| New Zealand white rabbit (Oryctolagus cuniculus) | 2 | F | Ivag | 0 | 2 | 0 | Not euthanized | Blood, Saliva, Vaginal swab, Urine |
N/A | |
| 6 | M | SC | 0 | 4 | 2 | 7 dpi, 28 dpi * | Blood, Saliva, Semen Urine |
Testes, Seminal vesicles |
||
| Hartley guinea pig (Cavia porcellus) | 8 | M | SC | 0 | 3 | 1 | 7 dpi | Blood, Saliva, Urine |
Testes, Cowper’s gland |
|
| MB | 3 | 1 | ||||||||
* for each timepoint, 2 inoculated rabbits and 1 sham-inoculated rabbit where euthanized, ** ante-mortem samples were collected every two days, *** no organs were collected from female rabbits, Ivag = intravaginal, SC = subcutaneous, MB = mosquito bite. Sham-inoculated animals (with 100 µL of PBS) were negative controls. N/A = non-applicable, ZIKV PRVABC59 = ZIKV strain PRVABC59 (ZIKV-PR; GenBank: KU501215), ZIKV 41525 = ZIKV strain DAK 41525 (GenBank: KU955591.1).