Table 3.

Study design for the inoculation of New Zealand white rabbits with ZIKV.

Animal ID	ZIKV Strain	Sex	Euthanized dpi
Rabbit 1	Zika PRVACB59	Male	7
Rabbit 2	Zika PRVACB59	Male	7
Rabbit 3	Mock	Male	7
Rabbit 4	Zika PRVACB59	Male	28
Rabbit 5	Zika PRVACB59	Male	28
Rabbit 6	Mock	Male	28
Rabbit 7	Zika PRVACB59	Female	N/A
Rabbit 8	Zika PRVACB59	Female	N/A

All animals were subcutaneously inoculated with 2.6 × 10⁶ PFU in 100 µL of virus or 100 µL of PBS (Mock). Females were not euthanized after 28 days; saliva, urine and vaginal swabs were negative by qRT-PCR (N/A = non-applicable). ZIKV PRVABC59 = ZIKV strain PRVABC59 (ZIKV-PR; GenBank: KU501215).