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. 2021 Aug 6;13(8):1217. doi: 10.3390/pharmaceutics13081217

Figure 3.

Figure 3

Presence of IS2 enhances the capture of IDLVs in DSBs. (A) Schematic diagram of the two constructs used in this study. (B) Workflow followed and experimental schema for IDLV gene trapping, 293T cell transduction with IDLV expressing GFP, followed by treatment with etoposide (8 µM). (C) Graph showing intracellular IDLV yield after transduction with the same multiplicity of infection (MOI = 4). (D) Representative experiment showing the increase in the percentage of eGFP expression, reflecting episomal relaxation due to etopside treatment, and the increased levels of eGFP labelling 36 d after transduction, which were further enhanced upon transduction with SE-IS2 (in-LTR). (E) IDLV capture by DSBs relative to the initial amount of viral genome episomes in the cells. The graphs represent mean of at least three separate experiments, while error bars indicate standard error of the mean (SEM); * = p < 0.01; two-tailed unpaired Student’s t-test.