FIG. 5.

Gln22 of Pax-5 is required for efficient assembly of ternary complexes with Ets-1. EMSA was performed with wild-type (wt) or mutated Pax-5(1–149) polypeptide and either the CD19 or mb-1 DNA probe, as indicated. Lysates in lanes pET were prepared from E. coli transformed with empty pET-11a vector. TC, ternary complexes; F, free probe.