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Recruitment of the Ets-1 ETS domain by Pax-2. (A) The paired domain of Pax-2 (residues 1 to 148) binds the mb-1 probe and recruits Ets-1. EMSA was performed with the mb-1 probe. Binding assays were performed with rabbit reticulocyte lysates programmed with synthetic RNA encoding the Pax-2 paired or Ets-1 ETS domain or with a translation without added RNA (No RNA). Both, Pax-2 and Ets-1 were both added after their separate translation; TC, ternary complexes; F, free probe.
