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PCR-RFLP digestion profiles. Undigested ITS2 amplicon and BauI restriction digestion profile for variable sequences of the ITS2 PCR amplicon of R. appendiculatus (B = undigested, C = digested), R. muehlensi (D = undigested, E = digested), R. simus (F = undigested, G = digested), R. maculatus (H = undigested, I = digested), R. microplus (J = undigested, K = digested), and R. decoloratus (L = undigested, M = digested) with 1 kb base pair ladder in A and N.
