Figure 3.

Impacts of csn5 disruption on radial growth under normal and stressful conditions. (A) Images (scale bar: 2 cm) for 8-day-old colonies grown at 25 °C on rich SDAY alone (control) or exposed to a 3-h heat shock at 42 °C after 2-day growth as well as minimal CDA alone (control) or supplemented with the indicated concentrations of chemical stressors (SBT, sorbitol; MND, menadione; CGR, Congo red; CFW, calcofluor white). (B) Relative growth inhibition (RGI) percentages of fungal colonies under the stressors. All colonies were initiated by spotting 1 μL aliquots of a 10⁶ conidia/mL suspension. (C) Total activities of superoxide dismutases (SOD) and catalases (CAT) quantified from the protein extracts of 3-day-old SDAY cultures. (D) Relative transcript (RT) levels of heat-responsive genes in the 3-day-old SDAY cultures of csn5 mutants with respect to the WT standard. The dashed line indicates a significant level of one-fold (50%) downregulation. * p < 0.05 or *** p < 0.001 in Tukey’s HSD tests. Error bars: SDs of the means from three independent replicates.