Table 1.
Primer sequences for RAA, plasmid, crRNA and ssDNA-FQ.
| Assay | Primer Name | Sequence (5′→3′) | Product Size (bp) |
|---|---|---|---|
| RAA | 529bp-RAA-F1 | GAAGGGACAGAAGTCGAAGGGGA | 171 |
| 529bp-RAA-R1 | GAAAAGCAGCCAAGCCGGAAACA | ||
| 529bp-RAA-F2 | TGGAGCCACAGAAGGGACAGAAGT | 184 | |
| 529bp-RAA-R2 | CAGGAAAAGCAGCCAAGCCGGAAA | ||
| 529bp-RAA-F3 | CAGAAGGGACAGAAGTCGAAGGGGA | 175 | |
| 529bp-RAA-R3 | AGGAAAAGCAGCCAAGCCGGAAACA | ||
| 529bp-RAA-F4 | GAGCCACAGAAGGGACAGAAGTCG | 186 | |
| 529bp-RAA-R4 | CCTCCAGGAAAAGCAGCCAAGCCG | ||
| Plasmid | 529bp-PF | GGAGGAAGACGAAAGTTG | 515 |
| 529bp-PR | ACAGTGCATCTGGATTCC | ||
| crRNA | crRNA1 | UAAUUUCUACUAAGUGUAGAUACTCGGGCCCAGCTGCGTCT | |
| crRNA2 | UAAUUUCUACUAAGUGUAGAUACAGGCAAGCTCGCCTGTGC | ||
| crRNA3 | UAAUUUCUACUAAGUGUAGAUCACCCUCCAGGAAAAGCAGCCA | ||
| crRNA4 | UAAUUUCUACUAAGUGUAGAUCTCGTGGTGATGGCGGAGAG | ||
| ssDNA-FQ | TgCas12a | 6FAM-CCGGAAAAAAAAAAAACCGG-BHQ1 |