Table 1.
Composition of the infusate.
| Amino Acid | Isotopic Labelling | Concentration of Amino Acid (mg/mL) | Molar Concentration |
|---|---|---|---|
| Valine | (1-13C) | 1.25 | 0.01 |
| Leucine | (1-13C) | 1.25 | 0.009 |
| Isoleucine | (1-13C) | 1.25 | 0.009 |
| Methionine | [Methyl-2H3] | 1.25 | 0.008 |
| Threonine | [U-13C4] | 2.5 | 0.02 |
| Phenylalanine | [1-13C] | 1.25 | 0.007 |
| Lysine | [4,4,5,5-2H4] | 2.5 | 0.011 |
| Histidine | [Ring 2-13C] | 2.5 | 0.012 |
| Tryptophan | [Indole-2H5] | 2.5 | 0.012 |
All nine stable isotope-labeled (13C or 2H) essential amino acids were dissolved in PBS (physiological saline solution) [35].