Figure 8.

Effects of PALO and/or MLA on hippocampal CA1 histopathological examination in HFFD/LPS-induced AD model. Photomicrograph of H&E stained sections from CA1 region of hippocampus of rats in (a) NFD reveals the normal histological architecture of this area with tightly packed normal pyramidal neurons having large vesicular nuclei (NPN). Sections of (b,c) AD model shows marked shrunken (SH) and necrosis (NC) of pyramidal neurons, flame-shaped neurofibrillary tangles (NFT) proliferation of glia cells (GL) and neurophagia (NP). Sections of (d) PALO, as well as (e) MLA treated groups show necrosis (NC) of some neurons and other neurons appeared normal (NPN), while sections of (f) PALO + MLA treated group shows restored histologically normal neurons (NPN) with necrosis of sporadic neurons (NC) (Scale bar 20 µm, × 600). Panel (g) represents collective CA1 damage scores presented in box and whisker and analyzed using Mann–Whitney test to compare between 2 groups. Panel (h) depicts individual CA1 alteration scores expressed as median (max-min) and analyzed using the Kruskal–Wallis test, followed by Dunnett’s post hoc test (p < 0.05), as compared from (*) NFD and (#) PALO + MLA treated group. PALO (0.1 mg·kg⁻¹; i.p) and/or MLA (5.6 mg·kg⁻¹; i.p) were administered 3 h after LPS injection and for 8 days.