Figure 3.

Analysis of transgene integration and expression by the PCR method (a,b); RT-PCR (c); the DbGluc1-HisTag detection technique (d) and SDS-PAGE detection of DbGluc1 activity (e). DbGluc1 (a) and neomycin phosphotransferase II (b) genes were detected in genomic DNA of transgenic plants with specific primers as PCR products of 580 and 500 bp, respectively. (c) RT-PCR analysis with primers designed within the DbGluc1 gene with the expected 129 bp length of the PCR product. (d) DbGluc1-His-tag protein expression examined using the InVision His-tag In-gel Stain detected as a single 52 kDa band with varying intensity in individual transformants. (e) Detection of β-1,3-glucanase activity on SDS-PAGE with laminarin following SDS removal.