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Overexpression of IRS-1 promotes proliferation and migration of NSCLC cells. (A) Validation of IRS-1 transcription (24 h) and protein (48 h) expressions after PCMV7.1-IRS-1 vector transfection in A549 and H1299 cells. (B) Proliferation analysis of A549 and H1299 cells after IRS-1 overexpression. (C) Colony forming analysis of A549 and H1299 cells after IRS-1 overexpression. (D) Wound-healing analysis of A549 and H1299 migration after IRS-1 overexpression. * p < 0.05; ** p < 0.01.
