Figure 2.

Phylogenetic relationships among 16S rRNA gene sequences of the 92 isolates from Shiraki Tsunesuke Sake Brewery, 6 isolates from Narimasa Sake Brewery, and 10 known species of Bacillus. A neighbor-joining tree was constructed with 1000 bootstrap replicates using MEGA X [18]. The evolutionary distances were computed using the maximum composite likelihood method [19], and are in the units of the number of base substitutions per site. All positions containing gaps and missing data were eliminated (complete deletion option). There were total 392 positions in the final dataset.