Figure 2.

Reactive oxygen species (ROS) production in bean leaf-disks subjected to different treatments: water (Mock, green line), 100 μM INA (INA, blue line), 1 μM flg22 (flg22, red line), or 1 μM flg22 after pre-treatment with 100 μM of INA (preINA + flg22, yellow line), and measured as relative light units (RLU) produced by Luminol reaction over the time. Total areas under the curves were integrated, and resultant values are represented at the right side of the panel. Data represent mean ± SE (n = 8) from one experiment representative of three independent ones with similar results. Statistically significant differences, indicated by letters, were achieved according to one-way ANOVA (p < 0.05), by post hoc Tukey test.