FIG. 6.

(A) Schematic representation illustrating the relative expression of gene products involved in sexual differentiation of the gonad. (B) Model depicting our results and the predicted effects on gene expression in the developing male gonad. The separate nuclear localization of WT1 isoforms is highlighted. The male-specific gene Sry is antagonized by the Dax-1 gene product (47), whereas MIS is activated via a synergistic effect of SF-1–WT1(−KTS) complexes, causing regression of the female anlagen (33). As a result of WT1-mediated activation, levels of DAX-1 are increased during normal early gonadal development. Once DAX-1 has accumulated to a certain threshold level, Sry activity is antagonized.