FIG. 5.

dnNFAT causes a dose-dependent inhibition of NFAT activity in Jurkat T cells. Increasing amounts (1, 3, 6, and 9 μg) of a dnNFAT expression vector were transfected in Jurkat T cells. The transcription activity of endogenous NFAT was detected with an NFAT-luciferase reporter plasmid. The effect of mutation of the PxIxIT motif by replacement of the Pro, Ile, and Thr residues with Ala (AxAxAA) was investigated. Luciferase activity was measured in cultures incubated without (−) or with (+) ionomycin (2 μM) and PMA (100 nM) (I+P). The data are presented as fold activation compared to an untreated control.