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. 2021 Jul 30;13(8):2664. doi: 10.3390/nu13082664

Figure 6.

Figure 6

Effect of DHA treatment on lipid droplet formation in A4-004N shControl and A4-004N shFABP7 cells. (A) A4-004N shControl and shFABP7 cells were cultured in medium supplemented with BSA control or 30 μM DHA. Cells were stained with Nile Red and images captured by confocal microscopy. DAPI was used to stain the nucleus. (B) Quantification of lipid droplets in A4-004N shControl and shFABP7 cells cultured in DHA-supplemented medium. The average intensity of Nile Red staining per cell was measured using raw images (n = 8, 15–30 cells/image) taken by confocal microscopy. *** indicates p < 0.001.