Figure 4.

γ-IFN-ELISPOT reactivity stratified by tumor inflammation score^HIGH (TIS) vs. TIS^LOW. Whiskers represent minimum and maximum TIS scores. Statistical analyses of TIS scores were performed using unpaired T-tests with Welch correction. (A) Volcano plot of p-value versus log2 fold change in the differential expression between TIS^HIGH and TIS^LOW. The test for differential expression was done by fitting the log2 normalized count to the response with linear model. The p-values were adjusted by the Benjamini and Yekutieli (BY) adjustment. Dots corresponding to genes that are significant at p < 0.5 (dashed line) are labeled in red. Solid line represents p < 0.10 (B). Overall survival relationship of Vigil treatment stratified by TIS^HIGH vs. TIS^LOW (C).