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. 2021 Aug 18;13(8):575. doi: 10.3390/toxins13080575

Figure 3.

Figure 3

Effect of the recombinant USCTX produced by cell-free expression on the viability of mouse neuroblasts (N2a cells). We tested all three IMAC elution fractions, namely the purification flow-through (E1), water elution (E2), and washing buffer elution (E3). The inhibitory effect was measured relative to the positive control (HPC; maximum inhibition induced by Triton X-100, lowest viability) and negative control (HNC; untreated cells in growth medium, zero inhibition, highest viability).