Figure 4.

PD-1 blockade inhibits tumor growth by decreasing proliferation and increasing apoptosis in TME. Tumor lysates from vehicle control, 3 mg/kg PV and 3 mg/kg TV were analyzed by Western blot with antibodies against PD-1, PD-L1, cleaved caspase-9, Ki-67. GAPDH was used as a loading control. Triplicate samples were loaded (n = 3 mice/group) and the signals were quantified using densitometric analysis and normalized with GAPDH protein expression. Results are shown as mean ± SEM.