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. 2021 Jul 28;13(8):529. doi: 10.3390/toxins13080529

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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SAW real-time interaction sensorgrams gained for binding of Stx1a and Stx2a toward DRM (A,C) and nonDRM fractions (B,D) prepared from replicate 2 of pHRPTEpiCs. The biosensor surface was coated with pooled DRMs (F1 to F3) (A,C) or nonDRM fraction F7 (B,D), and the interaction of Stx1a and Stx2a was portrayed for the representative channel 3 of the biochips as association and dissociation curves using increasing toxin concentrations as indicated. Start: begin of toxin exposure; stop: end of toxin exposure.