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. 2021 Aug 10;13(8):556. doi: 10.3390/toxins13080556

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A) The representative plasmid for the expression of r3FTX fused with DsbC at the N-terminus. The purity of the acquired rsNTX (B,C), rLNTX (D,E), and rCTXA3 (F,G) was confirmed by SDS-PAGE; the gels were stained with Coomassie blue, and the proteins were assayed by Western blotting with antivenom antibodies. Lane M: prestained protein markers; lanes 1 and 5: induced total lysate (total lysate after induction); lanes 2 and 6: total lysate before the induction; lanes 3 and 7: soluble fraction after cell disruption; lanes 4 and 8: purified recombinant 3FTX (indicated by an arrow).