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. 2021 Aug 3;9(8):850. doi: 10.3390/vaccines9080850

Figure 2.

Figure 2

Replication of scPR8-RBD-M2 in MDCK cells. (A) MDCK and MDCK-HA cells were infected with scPR8-RBD-M2 and subjected to immunofluorescence assay. α-NP and -RBD antibodies were used to detect the presence of influenza NP and SARS-CoV-2 RBD in infected cells. Nuclear staining by DAPI dye was indicated. (B) scPR8-RBD-M2, scPR8-mCherry-M2 and parental PR8 viruses were inoculated in MDCK and MDCK-HA cells at MOI of 0.01. The viruses were then titrated at indicated time points. (C) MDCK-HA cells were infected with the scPR8-RBD-M2 virus at varied MOIs. Cell supernatants were collected to measure virus titers by TCID50 assay at indicated time points. Error bars represent the mean ± standard error of mean. ** p < 0.01, *** p < 0.001, **** p < 0.0001.