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. 2021 Aug 3;9(8):850. doi: 10.3390/vaccines9080850

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Presentation of RBD on the membrane of infected cells and viral particles. (A) Confocal microscopy displayed surface expression of a membrane anchored-RBD in infected MDCK-HA. MDCK-HA cells were infected with scPR8-RBD-M2 and prepared under non-permeabilized and permeabilized conditions for IFA. The cells were probed with rabbit anti-spike RBD antibody. Goat anti-rabbit IgG antibodies conjugated with Alexa flour 488 was used as secondary antibody. (B) scPR8-RBD-M2 and scPR8-mCherry-M2 viruses were centrifuged through 20% glycerol in PBS and re-suspended with PBS. The purified viruses were subjected to (B) Western blot analysis, (C) negative staining and (D) immuno-labeling determined by transmission electron micrograph (TEM). The virus particles were labelled with rabbit anti-RBD monoclonal antibodies conjugated to 10-nm gold particles.