Figure 4.

Flow cytometry analysis of immune cells in lungs from C57BL/6J mice following a single intranasal dose of AdCOVID. C57BL/6J mice were given a single intranasal administration of vehicle (Ctrl) or 3.35 × 10⁸ ifu AdCOVID. Lung cells were isolated from the vaccinated mice at the timepoints indicated (10 mice/timepoint) and analyzed individually by flow cytometry using markers of (A) innate immune cells or (B) B and Tfh-like cells as described in the Materials and Methods. Results are expressed as cell number. Different Y-axis scales are used across the graphics. Data are the mean response ± SD. Statistical analysis was performed with a Mann–Whitney test: *** p < 0.001; **** p < 0.0001.