FIG. 1.

Purification of DEF-1 protein. (A) Calf brain lysates were passed over the GST, Src SH3, or Src SH3SH2 affinity column, and associated proteins were electrophoresed on a 10% polyacrylamide gel and visualized by silver stain. Molecular size markers in kilodaltons are indicated on the left. (B) Bound proteins from panel A were eluted with free glutathione and passed over an ATP-agarose column. The associated proteins were electrophoresed on a 5% polyacrylamide gel and visualized by silver staining.