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. 2021 Aug 10;13(8):558. doi: 10.3390/toxins13080558

Table 1.

Descriptive statistics of RNA-sequencing data for NN-SL venom gland transcriptome.

Parameter Sequencing Output
Total raw reads 54,477,234
Total clean reads 51,595,008
Total clean nucleotides (nt) 4,643,550,720
Q20 (%) 98.50
N (%) 0.01
GC (%) 44.62
Contigs assembly statistics 106,637
Total length (nt) 28,724,569
Mean length (nt) 269
N50 length (bp) 361
Unigenes assembly statistics 53,595
Total length (nt) 25,919,321
Mean length (nt) 484
N50 length (bp) 676

Q20 is the proportion of nucleotides with quality value larger than 20; N percentage is the proportion of unknown nucleotides in clean reads; GC percentage is the proportion of guanidine and cytosine nucleotides among the total nucleotides; N50 is the shortest contig length to cover 50% of the transcriptome.