Table 1.
Parameter | Sequencing Output |
---|---|
Total raw reads | 54,477,234 |
Total clean reads | 51,595,008 |
Total clean nucleotides (nt) | 4,643,550,720 |
Q20 (%) | 98.50 |
N (%) | 0.01 |
GC (%) | 44.62 |
Contigs assembly statistics | 106,637 |
Total length (nt) | 28,724,569 |
Mean length (nt) | 269 |
N50 length (bp) | 361 |
Unigenes assembly statistics | 53,595 |
Total length (nt) | 25,919,321 |
Mean length (nt) | 484 |
N50 length (bp) | 676 |
Q20 is the proportion of nucleotides with quality value larger than 20; N percentage is the proportion of unknown nucleotides in clean reads; GC percentage is the proportion of guanidine and cytosine nucleotides among the total nucleotides; N50 is the shortest contig length to cover 50% of the transcriptome.