. 2021 Aug 10;13(8):558. doi: 10.3390/toxins13080558

Table 2.

Overview of toxin gene families and transcript abundances (%) in venom gland transcriptome of NN-SL.

Gene ID	Protein Family/ Subfamily	Annotated Accession (NCBI/Uniprot)	Species	Coverage ^A (%)	Transcript ^B Abundance (%)
Three-finger toxins (3FTX)					87.54%
Short-chain 3FTX: Short α-NTX (SNTX)					1.78%
NN-3FTX01	Short neurotoxin 2	P62376	Hydrophis cyanocinctus	13–79	0.11%
NN-3FTX02 *	Cobrotoxin	P60770	Naja atra	1–82 (98.80)	0.69%
NN-3FTX03 *	Cobrotoxin-b	P80958	Naja atra	1–82 (100)	0.97%
Short-chain 3FTX: Orphan Group I					0.61%
NN-3FTX04	Neurotoxin-like protein NTL2	Q9W717	Naja atra	1–75	0.61%
Short-chain 3FTX: Muscarinic toxin-like protein (MTLP)					1.37%
NN-3FTX05 *	Neurotoxin homolog NL1	Q9DEQ3	Naja atra	6–86 (94.19)	0.96%
NN-3FTX06	Muscarinic toxin-like protein 2	P82463	Naja kaouthia	1–46	0.41%
NN-3FTX07	Haditoxin	A8N286	Ophiophagus hannah	36–84	0.001%
Short-chain 3FTX: Cytotoxin/cardiotoxin (CTX)					77.33%
NN-3FTX08 *	Cytotoxin 5	Q98961	Naja atra	1–81 (100)	77.33%
Long-chain 3FTX: Long α-NTX (LNTX)					1.10%
NN-3FTX09 *	Long neurotoxin 7	O42257	Naja sputatrix	12–88 (97.78)	1.00%
NN-3FTX10	Long neurotoxin 1	Q8UW29	Hydrophis hardwickii	12–91	0.01%
NN-3FTX11	Long neurotoxin-like OH-31	Q53B55	Ophiophagus hannah	20–59	0.08%
NN-3FTX12	Long neurotoxin-like OH-31	Q53B55	Ophiophagus hannah	5–59	0.003%
Non-conventional 3FTX: Weak neurotoxin (WTX)					5.35%
NN-3FTX13	Probable weak neurotoxin NNAM1	Q9YGI2	Naja atra	15–86	5.35%
Phospholipase A₂ (PLA₂)					2.08%
NN-PLA01	Basic phospholipase A₂	P00610	Hydrophis schistosus	23–119	0.01%
NN-PLA02	Basic phospholipase A₂	P00610	Hydrophis schistosus	23–119	0.002%
NN-PLA03	Acidic phospholipase A₂ 57	Q8UW31	Hydrophis hardwickii	51–135	0.01%
NN-PLA04 *	Acidic phospholipase A₂ 2	P00597	Naja kaouthia	1–146 (100)	2.05%
NN-PLA05	Basic phospholipase A₂ 73	Q8UW30	Hydrophis hardwickii	1–56	0.01%
Snake venom metalloproteinase (SVMP)					3.80%
NN-SVMP01	Scutatease-1	B5KFV7	Notechis scutatus	337–388	0.04%
NN-SVMP02	Zinc metalloproteinase mocarhagin	Q10749	Naja mossambica	502–568	0.03%
NN-SVMP03	Zinc metalloproteinase mocarhagin	Q10749	Naja mossambica	191–241	0.03%
NN-SVMP04	Zinc metalloproteinase mocarhagin	Q10749	Naja mossambica	143–195	0.03%
NN-SVMP05	Zinc metalloproteinase-disintegrin cobrin	Q9PVK7	Naja kaouthia	159–499	0.27%
NN–SVMP06	Zinc metalloproteinase-disintegrin cobrin	Q9PVK7	Naja kaouthia	159–600	0.16%
NN-SVMP07	Zinc metalloproteinase-disintegrin cobrin	Q9PVK7	Naja kaouthia	159–600	0.13%
NN-SVMP08	Zinc metalloproteinase-disintegrin atrase-B	D6PXE8	Naja atra	322–369	0.01%
NN-SVMP09	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	456–607	0.10%
NN-SVMP10	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	456–607	0.10%
NN-SVMP11	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	456–607	0.15%
NN-SVMP12	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	403–607	0.13%
NN-SVMP13	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	403–607	0.17%
NN-SVMP14	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	456–607	0.28%
NN-SVMP15	Zinc metalloproteinase-disintegrin atrase-A	D5LMJ3	Naja atra	1–229	1.25%
NN-SVMP16	Hemorrhagic metalloproteinase-disintegrin kaouthiagin	P82942	Naja kaouthia	6–64	0.03%
NN-SVMP17	Zinc metalloproteinase-disintegrin-like atragin	D3TTC2	Naja atra	527–613	0.80%
NN-SVMP18	Hemorrhagic metalloproteinase-disintegrin kaouthiagin	P82942	Naja kaouthia	130–201	0.07%
Cysteine-rich secretory protein (CRISP)					0.35%
NN-CRISP01 *	Natrin-1	Q7T1K6	Naja atra	1–239 (100)	0.35%
Snake venom serine protease (SVSP)					1.56%
NN-SVSP01 *	Serine protease harobin	Q5MCS0	Hydrophis hardwickii	1–265 (96.60)	1.56%
NN-SVSP02	Serine protease HTRA1	V8NGT0	Ophiophagus hannah	79–304	0.001%
NN-SVSP03 *	Serine protease 23	V8N8N4	Ophiophagus hannah	8–372 (98.12)	0.001%
Cobra venom factor (CVF)					0.26%
NN-CVF01	Cobra venom factor	Q91132	Naja kaouthia	1586–1642	0.13%
NN-CVF02 *	Cobra venom factor	Q91132	Naja kaouthia	1–1592 (96.96)	0.10%
NN-CVF03	Cobra venom factor	Q91132	Naja kaouthia	1197–1592	0.02%
NN-CVF04	Cobra venom factor	Q91132	Naja kaouthia	1–640	0.01%
Kunitz-type serine protease inhibitor (KSPI)					0.50%
NN-KSPI01 *	Putative Kunitz-type serine protease inhibitor	B2BS84	Austrelaps labialis	1–252 (98.81)	0.02%
NN-KSPI02 *	Kunitz-type protease inhibitor	U3FZD6	Micrurus fulvius	1–511 (99.61)	0.004%
NN-KSPI03	Protease inhibitor 3	C1IC51	Walterinnesia aegyptia	1–67	0.17%
NN-KSPI04	Kunitz-type serine protease inhibitor NACI	Q5ZPJ7	Naja atra	43–81	0.30%
Phosphodiesterase (PDE)					0.08%
NN-PDE01 *	Snake venom phosphodiesterase	A0A2D0TC04	Naja atra	21–850 (97.65)	0.08%
5’-Nucleotidase (5’-NUC)					0.06%
NN-NUC01 *	Ecto-5’-nucleotidase	A0A194AS98	Micrurus tener	1–569 (99.13)	0.05%
NN-NUC02 *	Ecto-5’-nucleotidase	A0A194AS98	Micrurus tener	1–536 (93.38)	0.008%
NN-NUC03 *	Ecto-5’-nucleotidase	A0A194AS98	Micrurus tener	1–536 (93.38)	0.004%
NN-NUC04	5’ nucleotidase	A6MFL8	Demansia vestigiata	134–559	0.001%
Acetylcholinesterase (AChE)					0.01%
NN-ACHE01 *	Acetylcholinesterase	Q92035	Bungarus fasciatus	1–566 (95.55)	0.004%
NN-ACHE02	Acetylcholinesterase	Q92035	Bungarus fasciatus	1–566 (95.55)	0.002%
Hyaluronidase (HYA)					0.002%
NN-HYA01	Hyaluronidase	V8PHI0	Ophiophagus hannah	26–71	0.001%
NN-HYA02	Hyaluronidase	A0A2D4JS30	Micrurus lemniscatus lemniscatus	89–208	0.0005%
NN-HYA03	Hyaluronidase	A0A2D4JS30	Micrurus lemniscatus lemniscatus	27–91	0.0003%
NN-HYA04 *	Hyaluronidase	A0A194APD1	Micrurus tener	1–440 (98.43)	0.0003%
Nerve growth factor (NGF)					2.88%
NN-NGF01 *	Venom nerve growth factor 2	Q5YF89	Naja sputatrix	1–241 (100)	2.88%
Vespryn (VES)					0.04%
NN-VES01 *	Ohanin	P83234	Ophiophagus hannah	1–181 (100)	0.03%
NN-VES02 *	Ohanin	P83234	Ophiophagus hannah	1–181 (100)	0.02%
Vascular endothelial growth factor (VEGF)					0.001%
NN-VEGF01 *	Vascular endothelial growth factor 1	U3F558	Micrurus fulvius	1–142 (95.95)	0.001%
NN-VEGF02 *	Vascular endothelial growth factor 1	U3F558	Micrurus fulvius	1–142 (95.95)	0.0003%
Dipeptidyl peptidase IV (DPP IV)					0.02%
NN-DPP01	Dipeptidyl peptidase 4	V8P9G9	Ophiophagus hannah	0.02%	0.02%
Phospholipase B (PLB)					0.01%
NN-PLB01 *	Phospholipase-B 81	F8J2D3	Drysdalia coronoides	1–553 (100)	0.01%
NN-PLB02	Phospholipase B-like	V8NLQ9	Ophiophagus hannah	146–196	0.001%
Snake C-type lectin (SNACLEC)					0.17%
NN-SNAC01 *	C-type lectin BfL-1	Q90WI8	Bungarus fasciatus	1–158 (100)	0.06%
NN-SNAC02 *	C-type lectin BfL-1	Q90WI8	Bungarus fasciatus	1–148 (98.67)	0.11%
NN-SNAC03	Venom C-type lectin mannose binding isoform 1 variant 3	D2YVK0	Tropidechis carinatus	57–158	0.001%
Cystatin (CYS)					0.01%
NN-CYS01	Cystatin	E3P6P4	Naja kaouthia	0.01%	0.01%
Aminopeptidase (AP)					0.004%
NN-AP01 *	Aminopeptidase	U3FZS8	Micrurus fulvis	1–992 (98.29)	0.003%
NN-AP02 *	Aminopeptidase	U3FZS8	Micrurus fulvius	1–992 (98.29)	0.001%
Waprin (WA)					0.001%
NN-WAP01	Waprin-Phi3	A7X4M7	Philodryas olfersii	33–80	0.001%
Natriuretic peptide (NP)					0.55%
NN-NP01	Natriuretic peptide Na-NP	D9IX97	Naja atra	34–77	0.41%
NN-NP02	Natriuretic peptide Na-NP	D9IX97	Naja atra	115–165	0.08%
NN-NP03	Natriuretic peptide Na-NP	D9IX97	Naja atra	25–80	0.06%
L-amino acid oxidase (LAAO)					0.08%
NN-LAAO01	L-amino-acid oxidase	Q4JHE3	Oxyuranus scutellatus scutellatus	1–517	0.04%
NN-LAAO02	L-amino-acid oxidase	Q4JHE3	Oxyuranus scutellatus scutellatus	1–517	0.02%
NN-LAAO03	L-amino-acid oxidase	Q4JHE3	Oxyuranus scutellatus scutellatus	1–517	0.02%

* Indicates full-length transcripts of NN-SL with >90% coverage to the annotated sequence. ^A indicates the length of coverage of toxin sequence when compared to the annotated sequence, and the value in bracket refers to the % coverage of full-length transcript of NN-SL. ^B Transcript abundance (in %) was based on FPKM (fragments per kilobase of transcript per million mapped reads) of toxin genes in NN-SL.