Skip to main content
. 2021 Jul 29;9(8):833. doi: 10.3390/vaccines9080833

Figure 1.

Figure 1

Sequencing of the variable regions of PEDV neutralizing monoclonal antibody, E10E-1-10. (A) The PCR reactions were conducted by using one general forward primer and three specific reverse primers, which target on variable lambda light chain (Lanes 1 and 2), variable kappa light chain (Lanes 3 and 4), and variable heavy chain (Lanes 5 and 6). Lanes 2, 4, and 6 were negative controls for each PCR reaction. Major bands sized about 500–600 bp were obtained from Lane 3 (the target amplicon of variable kappa light chain was labeled with arrowhead) and Lane 5 (the target amplicon of variable heavy chain was labeled with asterisk) and sent for sequencing. (B) Deduced amino acid sequences and the predicted FRs and CDRs of the variable kappa light chain and variable heavy chain.